搜索:  
Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells  
Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells
资料类型: PDF文件
关键词: Cell-penetrating  peptides  Endocytosis  Heparan  sulfate  proteoglycans  Macropinocytosis  
Nona-arginine  
资料大小: 1001k
所属学科: 功能高分子
来源: 来源网络
简介:
The application of cell-penetrating peptides (CPPs) for delivering various cargo molecules with biologicalfunctions into cells has gained much attention in recent years. However, the internalizationmechanisms and delivery properties of CPP–cargo remains controversial. In this study, low- and highmolecular-weight cargoes attached to arginine-rich CPPs were employed: the former was the fluoresceinisothiocyanate-labeled nona-arginine (CPP–FITC), and the latter was the fluorescently labeled nonaarginine–avidin complex (CPP–avidin). We measured the intracellular trafficking of CPP–FITC andCPP–avidin in four cancer cell lines in a series of microenvironments altered by the presence or absenceof serum, different temperatures and different incubation times. The results revealed that CPP–cargodelivery exhibited no specificity toward any cell line, but the levels were found to be related to cell type and cargo. Furthermore, their endocytic mechanisms were investigated via incubation with relatedendocytic inhibitors. Two different types of CPP–cargo were required to cross the plasma membraneto bind to cell surface-associated heparan sulfate proteoglycans in a time-dependent manner. CPPs andsmall cargoes attached to CPP may enter cells rapidly via direct translocation in addition to the endocyticroute. Translocation of large components linked to CPP tended to be mediated by macropinocytosis in anenergy-dependent manner with slower rates for larger compounds. In contrast, the clathrin-dependentpathway is not essential to the translocation of either type of CPP–cargo.
上传人: shinianqiu
上传时间: 2017-4-14 12:51:16
下载次数: 0
消耗积分: 0  
立即下载:
1人
1人
1人
友情提示:下载后对该资源进行评论,即可奖励2分。
报告错误:  1.下载无效  2. 资料明显无价值  3. 资料重复存在

相关评论 共有0人发表评论 更多评论
你还没有登录,无法发表评论,请首先 登录 注册
免责声明:本站部分资源由网友推荐,来自互联网,版权属于原版权人,如果不慎侵犯到您的权利,敬请告知,我们会在第一时间撤除。本站中各网友的评论只代表其个人观点,不代表本站同意其观点。