writer：Peihong Xue, Wendong Liu, Zhongyi Gu, Xingchi Chen, Jingjie Nan, Junhu Zhang, Hongchen Sun, Zhanc
keywords：gradient biomaterials, colloidal lithography, well-de?ned ECM, inducing cellular behavior, multifunctional substrate
source：期刊
specific source：ACS Appl. Mater. Interfaces
Issue time：2019年

Gradient biomaterials have shown enormous

potential in high-throughput screening of biomaterials and

material-induced cell migration. To make the screening

process more rapid and precise, improving the regularity of

morphological structure and chemical modi?cation on

gradient biomaterials have attracted much attention. In this

paper, we present a novel fabrication strategy to introduce

ordered nanopattern arrays into gradient biomaterials, through

combining surface-initiated atom transfer radical polymer-

ization and inclined reactive-ion etching based on colloidal

lithography. Graded protein/poly(ethylene glycol) (PEG)

nanopattern arrays on a quartz substrate were fabricated and

applied to a?ect the behaviors of cells. Owing to the

continuously changed ratio of two di?erent components, the corresponding cell adhesion density along the substrate showed

obvious graded distribution after culturing for 24 h. Meanwhile, the cytoskeleton showed obvious polarization after culturing for

7 days, which is parallel with the direction of gradient. Additionally, oriented migration was generated when mouse MC3T3-E1

cells were cultured on the graded protein/PEG nanopattern arrays. On the basis of the ordered and well-de?ned nanopatterns,

the correlation between the extracellular matrix and corresponding expressions generated by di?erent stimuli can be

investigated.