writer：2) Wendong Liu, Xueyao Liu, Peng Ge, Liping Fang, Siyuan Xiang, Xiaohuan Zhao, Huaizhong Shen, and B
keywords：polymer brush, DNA patterns, nanocone arrays, sensing, hierarchical-multiplex
source：期刊
specific source：ACS Appl. Mater. Interfaces 2015, 7, 24760?24771
Issue time：2015年

This paper provides a facile and cost-e?cient

method to prepare single-strand DNA (ssDNA) nanocone arrays

and hierarchical DNA patterns that were mediated by poly(2-

hydroxyethyl methacrylate) (PHEMA) brush. The PHEMA brush

nanocone arrays with di?erent morphology and period were

fabricated via colloidal lithography. The hierarchical structure was

prepared through the combination of colloidal lithography and

traditional photolithography. The DNA patterns were easily

achieved via grafting the amino group modi?ed ssDNA onto the

side chain of polymer brush, and the anchored DNA maintained

their reactivity. The as-prepared ssDNA nanocone arrays can be applied for target DNA sensing with the detection limit reaching

1.65 nM. Besides, with the help of introducing micro?uidic ideology, the hierarchical-multiplex DNA patterns on the same

substrate could be easily achieved with each kind of pattern possessing one kind of ssDNA, which are promising surfaces for the

preparation of rapid, visible, and multiplex DNA sensors.