Fabrication of hydrophilic-oleophobic responsive membranes is greatly vital to satisfy the requirements of oilwater separation devices and oil droplet manipulation yet remains challenging due to the limitations imposed by surface tension theory. Herein, a seed-assisted in situ growth approach was employed to fabricate a hierarchical Zeolitic Imidazolate Framework-L (ZIF-L) layer, upon which a sodium perfluorooctanoate (PFOA-Na) solution was applied on the ZIF-L surface, thereby obtaining a highly responsive hydrophilic-oleophobic membrane

(ZIF-L-F). The ZIF-L-F membrane possesses ultra-slippery properties with water as a natural lubricant, finally displaying a remarkably high oily liquid repellency with an extremely low contact angle hysteresis (CAH<3?), due to the instantaneous molecular structure reversal of the embedded PFOA-Na. Benefiting from its selective wettability towards water and oil, the ZIF-L-F can be easily applied to stainless steel mesh to get an oilwater separation membrane with high efficiency above 97 %. Also, this ZIF-L-F membrane exhibited excellent antibacterial activities (≥98 %) against gram-negative bacteria, due to its mechano-bactericidal activity of the ZIF-L nano-blade structures. These findings support the development of functional membranes that can highly maintain oil-water separation while effectively avoiding bacterial contamination in environmental protection and water treatment applications.