As a type of room temperature phosphorescence (RTP) material, carbon dots (CDs) always show short lifetime and low phosphorescence efficiency. To counter these disadvantages, several strategies, such as embedding in rigid matrix, introducing of heteroatom, crosslink-enhanced emission, etc., are well developed. Consequently, lots of CDs-based RTP materials are obtained. Doping of CDs into various matrix is the dominant method for preparation of long-lived CDs-based RTP materials so far. The desired CDs@matrix composites always display outstanding RTP performances. Meanwhile, matrixfree CDs and carbonized polymer dots-based RTP materials are also widely developed. Amounts of CDs possessing ultra-long lived, multiple colored, and dynamic RTP emission are successfully obtained. Herein, the recent progress achieved in CDs-based RTP materials as well as the corresponding efficient strategies and emission mechanisms are summarized and reviewed in detail. Due to CDs-based RTP materials possess excellent chemical stability, photostability and low biological toxicity, they exhibit great application potential in the fields of anti-counterfeiting, data encryption, and biological monitoring. The application of the CDs-based RTP materials is also introduced in this review. As a promising functional material, development of long wavelength RTP emitting CDs with long lifetime is still challengeable, especially for the red and near-infrared emitting RTP materials.