A novel technique is reported for preparing wafer-scale polymer brush nanopatterns by combining

colloidal lithography with surface-initiated atom-transfer radical polymerization. The

nanoarchitecture parameters of the polymer patterns can be fully controlled by tuning the experimental

conditions and polymer nanopatterns with a lateral resolution down to 100 nm are successfully

achieved. In addition, by coupling this technique with photolithography, hierarchical polymer brush

nanopatterns have been fabricated. Signi?cantly, the in?uences of nanodot morphologies on the

feature sizes of the polymer nanopatterns are explained in detail. More importantly, complex protein

patterns are prepared by conjugating proteins on the polymer brush nanopatterns. The proteins are

covalently immobilized on the polymers and exhibit 3D distribution without denaturation. Cells can

adhere quickly to the ?bronectin patterns and elongate along the stripes of the protein patterns.