This paper presents a versatile way to prepare multiscale and gradient

patterns of proteins. The protein patterns are fabricated by conjugating proteins covalently

on patterns of polymer brush that are prepared by techniques combining colloidal

lithography with photolithography, and two-step colloidal lithography. Taking advantages

of this technique, the parameters of protein patterns, such as height, diameters, periods, and

distances between two dots, can be arbitrarily tuned. In addition, the protein patterns with

varies of architectures, such as microdiscs, microstripes, microrings, microtriangles,

microgrids, etc., consisting of protein nanodots, are prepared and the sample size is up to 4

cm2. The as-prepared patterns of ?bronectin can promote the cell adhesion and cell

location.