S. Y. Lee

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Bioinformatics Research Center, and Center for Ultramicrochemical Process Systems, Department of BioSystems, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea

Nanobiotechnology is a new discipline that fuses biological research and nanotechnological research in such areas as molecular self-assembly, spatial positioning, microconstruction and biocomposite fabrication, and offers exciting possibilities for the miniaturization of bioanalytical system. One of the important research topics is to develop a platform technology that allows studying biomolecular interactions on solid supports. Obviously, it is prerequisite to develop a method to immobilize biomolecules specifically and robustly on the desired regions of the solid supports while allowing efficient biomolecule-biomolecule interactions. In this sense, polyhydroxyalkanoates (PHAs) are attractive biomaterials for the fabrication of novel nano and/or micro-scaled structures and well-defined surfaces because of thermoplastic or elastomeric properties. We have developed a proprietary technique using the PHA depolymerase binding domain as a capture ligand for selective immobilization of any proteins and/or peptides onto the patterned or non-patterned surface of PHA. In one example, microcontact printing was employed to develop a well defined pattern of fusion proteins with 50 mm repeated stripes or circles on PHA. In another example, we developed a novel microbead-based assay system suitable for the analysis of protein-protein interactions. Direct contact printing of biomolecules onto the PHA chip could also be realized. Detailed results along with potential applications will be presented.

This work was supported by the KOSEF through the Center for Ultramicrochemical Process Systems, and also by the IMT-2000 project through the B-SAN.
Further support by LG Chemicals Chair Professorship is appreciated.

论文来源：International Symposium on Biological Polyesters ,Auguest 22-27, 2004