writer：Lingjie Song, Jie Zhao, Jing Jin, Jiao Ma, Jingchuan Liu, Shifang Luan*, Jinghua Yin*
keywords：Anti-bioadhesion Antibody immobilization Photografting polymerization
source：期刊
Issue time：2014年
A polymeric platform for immunodiagnostic bioassay was constructed based on biostable polymeric support and two functional monomers, low-fouling methacryloyloxyethyl phosphorylcholine (MPC) and acrylic acid (AAc), by a photografting polymerization approach. Covalent binding of antibody to pAAc chains was achieved by activating carboxyl with NHS/EDC partner. The resultant surface showed obvious suppression of nonspecific protein adsorption and platelet adhesion relative to the control sample, exhibiting good anti-bioadhesion performances. Based on the polymer-supported matrix, a highly sensitive antibody–antigen specific recognition was confirmed in both native plasma and diluted human plasma due to the enhanced antibody loading capacity and lowered bioadhesion as compared to the reference.