The controllable preparation of porous PLGA microspheres by the oil/water emulsion method and its application in 3D culture of ovarian cancer cells
作者：Tianzhu Zhang*, Qiying Zhang, Junsong Chen, Kun Fang, Jun Dou, Ning Gu*
关键字：PLGA Smooth microsphere,Porosity,3D culture,Ovarian cancer cells
论文来源：期刊
具体来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2014, 452: 115–124
发表时间：2014年
In this study, we prepared surface-smooth and porous poly(lactic-co-glycolic acid) (PLGA) microsphereswith different diameters, different sizes of pores and different densities of pores using a simple oil/wateremulsion method and varying the preparation conditions, including the molecular weight of PLGA, thecomponents of the PLGA and the addition of porogen. The surface-smooth nonporous and porous PLGAmicrospheres were also further modified with collagen I. The characteristics of these PLGA microspheres,including original and collagen I-coated PLGA microspheres, were evaluated in a three-dimensional (3D)culture of ovarian cancer HO8910 cells. The HO8910 cells can growth better on original porous andcollagen I-coated PLGA microspheres, and express remarkably E-cadherin. These results indicate thatporous PLGA microspheres and collagen-coated PLGA microspheres are promising candidates as ovariancancer cell culture microcarriers for pathological study and high-throughput antitumor drug screening.