writer：Shuaishuai Yuan, Yong Shen, Zhibo Li*
keywords：poly(4-hydroxybutyrate) (P4HB), double-emulsion solvent evaporation method, open porous microsphere, bone tissue engineering, bone defect
source：期刊
specific source：ACS Applied Bio Materials
Issue time：2021年

Delivering injectable microspheres in a minimally invasive way to repair

complexly shaped tissue defects renders them attractive for clinical use. Especially, open

porous microspheres that provide sufficient internal space for cell proliferation and

nutrient diffusions can efficiently aid to completing reconstructions of tissue defects. In

this work, chemically synthesized and biodegradable poly(4-hydroxybutyrate) (P4HB),

which is the U.S. FDA-approved polyhydroxyalkanoate (PHA), was employed for

fabricating open porous microspheres using a double-emulsion solvent evaporation

method. The influences of fabrication parameters were discussed. It was found that the

P4HB-based cell-free and growth factor-free open porous microspheres can enhance

osteoblast differentiation of adipose-derived stem cells in vitro and accelerate rat calvarial

bone-defect healing in vivo. These results demonstrated that the injectable open porous

P4HB microspheres present a remarkable potential in bone tissue regeneration.