writer：Xiaohan Wang, Xiaotao Zhao, Kai Zheng, Xuhong Guo, Yunfeng Yan,* and Yisheng Xu
keywords：nanoparticle, protein detection
source：期刊
specific source：Langmuir
Issue time：2019年

Quantitative detection of protein biomarkers is crucial to

medical diagnosis. Fluorescent probes have been frequently used for

protein detection, but they suffered from various weaknesses such as lack

of versatility. In particular, most of the reported probes were not capable

of simultaneous qualitative and quantitative detection for various

proteins. In this paper, we developed novel nanoparticle array-based

near-infrared (NIR) ratiometric probes for potent protein analysis, in

which the specific protein was able to be distinguished and quantitated

within a group of 11 common proteins. The activity of β-galactosidases

(β-gal) was temporarily inhibited by the adsorption to magnetic

nanoparticles and restored to certain content by replacement with

detected proteins, leading to distinctive readout of the enzymeactivatable NIR probe (DCM-β-gal). The readout of the sensor array

against 11 proteins, as verified by isothermal titration calorimetry, was processed and transformed into canonical factors with the

help of linear discrimination analysis. Moreover, the ratiometric signals of DCM-β-gal were translated to quantitatively detect

proteins within the concentration range of 0-100 μg/mL. Based on clear differentiation within both two-dimensional and

three-dimensional plots, different proteins could be detected with 100% accuracy with their concentration simultaneously

determined, which endowed the sensing system with great potential in clinical diagnosis