writer：Jin Gu,1, ? Yajuan Su,2, ? Peng Liu, 3 , * Peng Li,2, 4, * Peng Yang1, *
keywords：Surface Coating; Antimicrobial; Biocompatibility; Protein Phase Transition; Lysozyme
source：期刊
specific source：ACS Applied Materials & Interfaces
Issue time：2017年

DOI: 10.1021/acsami.6b13552

The use of antimicrobial materials e.g. silver nanoparticles, has been a cause for concern because they often exert adverse effect on environmental and safety during their preparation and use. In this study, we report a class of green antimicrobial coating based on a supramolecular assembly of a protein extracted from daily food, without the addition of any other hazard agents. It is found that a self-assembled nanofilm by mere hen egg white lysozyme has durable in vitro and in vivo broad-spectrum antimicrobial efficacy against Gram-positive/negative and fungi. Such enhanced antimicrobial capability over native lysozyme is attributed to a synergistic combination of positive charge and hydrophobic amino acid residues enriched on polymeric aggregates in the lysozyme nanofilm. Accompanied with high antimicrobial activity, this protein-based PTL material simultaneously exhibits the integration of multiple functions including antifouling, antibiofilm, blood compatibility and low cytotoxicity due to the existence of surface hydration effect. Moreover, the bio-inspired adhesion mediated by the amyloid structure contained in the nanofilm induces robust transfer and self-adhesion of the material onto virtually arbitrary substrates by a simple one-step aqueous coating or solvent-free printing in one minute, thereby allowing an ultrafast route into practical implications for surface functionalized commodity and biomedical devices. Our results demonstrate that the application of pure proteinaceous substance may afford a cost-effective green biomaterial that has high antimicrobial activity and low environmental impact.