writer：Peng Yang
keywords：Protein Self-assembling
source：期刊
specific source：Macromolecular Bioscience
Issue time：2012年
When lysozyme is dissolved in a neutral HEPES buffer solution (pH?=?7.4) with 0.001-0.050?M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue" that binds strongly and quickly onto non-fouling coatings. This binding is highly selective towards lysozyme, and excludes synthetic, chemical/physical activation/deactivation (blocking) steps. By using biotinylated lysozyme, such a phase transition quickly creates a perfect biotinylated surface on non-fouling surfaces for avidin binding, showing great potential for the development of low-cost and practical biochips.