writer：Wang, M.; Xing, J.; Sun, Y.; Guo, L.; Lin, B.; Yang, H.*
keywords：enzyme, polysiloxane
source：期刊
specific source：RSC Advances, 2018, 8, 11843-11849.
Issue time：2018年

Chemically

incorporation of enzymes onto polymeric materials has recently attracted

intense scientific attention. Cross-linked polysiloxane gels as a typical

super-hydrophobic support, is a good candidate for supporting enzymes in

low-water organic medium to efficiently catalyze peptide synthesis because the

hydrophobic polysiloxane matrice can prevent water from attacking the

acyl-enzyme intermediate, which is beneficial for the shift in equilibrium to

peptide formation. In this work, we develop a facile strategy to photoimmobilize

olefin-functionalized chymotrypsin onto cross-linked polysiloxane gels via UV-initiated thiol-ene click

chemistry. The impacts of water addition amount, heat-treatment and recyclability

of the immobilized chymotrypsin influencing on the peptide synthesis efficiency

are investigated. Compared with the native chymotrypsin,

polysiloxane-immobilized chymotrypsin showed advantageous catalytic activity,

higher thermal stability and superior recyclability.