项目类别：国家自然科学基金
项目编号：21574050
项目规模：面上项目
参与人员：杨光（主持）、石志军、黄芳、李莹、黄亚佳、刘莉、李思祥、曾迪
起止日期：2016.01-2019.12
  生物体产生的内源信号在发育、再生以及自我修复过程起着举足轻重的作用。本项目 采用已建立的生物制造过程控制的方法，在生物合成过程中经分子模板和微流控调控葡糖 醋杆菌的定向运动，此外通过构建趋光型葡糖木醋杆菌工程菌株，控制纤维素纤维的组装 与排列，光控合成具有特定规则图案的三维的纤维素材料，为组织细胞提供多层次高度精 细结构的组织工程支架，诱导其与支架的定向黏附和聚集。进一步通过电活性修饰，模拟 生物体的内源生物电信号，引导和调控成纤维细胞、神经元细胞、内皮细胞的分布、迁移 、增殖、分化等。本项目针对“调控内源信号实现无损定向诱导皮肤组织的修复与再生” 的关键科学问题。采用在有序微纳界面上模拟内源电信号刺激的方法，构建基于有序微纳 界面与生物电信号的定向诱导组织修复与再生的模型，在分子、细胞、组织等水平阐明微 纳界面诱导和内源生物电信号的协同作用机制，为实现皮肤组织的定向修复与再生提供科 学依据。
  Endogenous electric signal is fundamentally important for development, regeneration, and wound healing. This project adopts the recently established bio-manufacturing and process control methods to control the directed movement of Gluconacetobacter xylinum through molecular template and microfluidics. In addition, by constructing engineered phototaxic Gluconacetobacter xylinum, assembly and arrangement of cellulose fibers is light controlled to achieve a specific three-dimensional micro-pattern, which will provide highly sophisticated multi-level tissue engineering scaffolds to induce directed cell adhesion and aggregation, for regenerative medicine purposes Further, the micro-patterned materials will be locally modified with conductive materials to simulate the endogenous electrical signaling to induce and modulate the distribution, migration, proliferation, and differentiation of fibroblasts or neurons. t. This project aims to solve the key scientific issue concerning how to achieve directed induction of skin repair and regeneration through modulation of endogenous signaling by simulating the endogenous electric signal on a patterned micro-/nano-surface and also to elucidate the underlying mechanisms of the additive effect of micro-/nano-surface and biological electric signal at molecular, cellular, and tissue level. This will provide a solid scientific basis for directed repair and regeneration of skin tissues.