writer：Rong-Min Wang, Nai-Pu He, Yu-Feng He, Yun-Tao Xie, Yun-Pu Wang,Eishun Tsuchida
keywords：chitosan; nanocomposites; biological applications of polymers; DNA; copper complexes
source：期刊
specific source：POLYMERS FOR ADVANCED TECHNOLOGIES
Issue time：2005年

Water-soluble low molecular weight chitosan of nanometer level and its copper complexes were prepared, and characterized by IR spectra, elemental analysis and gel permeation chromatography (GPC). The modes and mechanism of these copper complexes interaction with DNA were studied by a fluorescent probe method and electrophoresis analysis. It is suggested that there are electrostatic and intercalation modes of copper complexes interacting with DNA. At first, the cationic complex electrostaticly binds to the negatively charged phosphate backbone of DNA, and then a portion of the complex intercalates between the base pairs on the DNA duplex strand.