writer：Xiaobo Liu, Yuhua Mao, Siyang Yu, Hao Zhang,* Kecheng Hu, Lingyu Zhu, Jianbing Ji and Jianli Wang*
keywords：Pickering; biocatalyst; biodiesel production
source：期刊
specific source：Green Chemistry
Issue time：2021年

       寻找一种绿色高效的催化剂对于生物柴油的生产来说是非常重要的。本研究制备了一种Pickering磁性界面生物催化剂(Fe3O4@PS-NH-lipase)，该催化剂能有效稳定大豆油与甲醇乳液从而实现了静态酯交换。通过调控甲醇/大豆油摩尔比、催化剂负载量，Pickering界面生物催化剂在酯交换反应中表现出了优异的的酶活性，30h内的转化率达到了89.3%。与Pickering协同催化(PAC，依靠纳米粒子与均相催化剂一起稳定Pickering乳剂)相比，Pickering界面催化（PIC，催化活性中心固定在载体上再稳定Pickering乳液)的催化活性提高了1.2倍，展现出了更显著的催化强化效果且回收的催化剂在循环5次后仍具有很好的催化活性。本文为生物活性物质有效回收和双相反应强化提供了一种绿色高效的策略。

Green and efficient catalysts for the production of biodiesel are highly important. In this study, a Pickering magnetic interface biocatalyst (Fe₃O₄@PS-NH-lipase) was prepared, which can effectively stabilize the soybean oil-in-methanol emulsion and achieve static transesterification. Choosing the appropriate methanol/soybean oil molar ratio and catalyst loading, Pickering interface biocatalyst exhibited outstanding enzymatic activity, reaching to a conversion rate of 89.3% after 30 h. Pickering interfacial catalysis (PIC, immobilizing catalytic centers on a carrier and combining with Pickering emulsion) shows more significant specific activity, leading to a 1.2-fold improvement in catalytic performance as compared to the Pickering assistant catalysis (PAC, relying on the use of nanoparticles for stabilizing Pickering emulsions together with a homogeneous catalyst). Moreover, the recycled catalyst still possesses significant catalytic activity after 5 cycles. This article provides a green and efficient strategy for the effective recovery of biologically active substances and the enhancement of biphasic reactions. 

Scheme 1 Schematic illustration of the recyclable Pickering magnetic interface biocatalyst for transesterification. 

DOI: 10.1039/D0GC03527K