writer：Wyatt, Mark F.; Schaeffer, Nicolas; Tan, Bien; Cooper, Andrew I.
keywords：MALDIT TOF
source：期刊
specific source：Journal of the American Society for Mass Spectrometry (2007), 18(8), 1507-1510.
Issue time：2007年

We evaluated several aq.-based sample prepn. protocols for the anal. of poly(methacrylic acid) (PMAA) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS).  The sample contained a pentaerythritol tetra(3-mercaptopropionate) end-group, and was characterized in pos. and neg. ion modes using 2,5-dihydroxybenzoic acid (DHB) and 2,4,6-trihydroxyacetophenone (THAP) matrixes.  The major series obsd. were the [M + Na]+ species, in pos. ion mode, and the [M - H]- species, in neg. ion mode.  The performance of DHB and THAP matrixes was comparable in pos. ion mode, but THAP outperformed DHB in neg. ion mode.  The use of ion-exchange beads (IXB) benefited the anal., while the addn. of sodium acetate (NaOAc) or trifluoroacetic acid (TFA) proved disadvantageous in pos. ion mode.