A chitin film containing basic fibroblast growth factor with a chitin-binding domain as wound dressings
作者：Yu Wang, Chuan Fu, Zhenxu Wu, Li Chen,Xuesi Chen,Yen Wei,Peibiao Zhang
关键字：Basic fibroblast growth factor; Chitin binding domain; Chitin; Vascularization; Wound dressing
论文来源：期刊
具体来源：Carbohydrate Polymers, 2017, 174：723-730
发表时间：2017年
Basic fibroblast growth factor (bFGF) can stimulate wound healing. However, consistent delivery of bFGF has many disadvantages. To decrease their instability and diffusible, we introduced chitin-binding domain (ChtBD) into bFGF. Two expression plasimids were constructed. The first one (named bFGF) contained bFGF (154 amino acids), the second (named ChtBD-bFGF) contained bFGF and the ChtBD (54 amino acids). ChtBD was derived from chitinase A1 (ChiA1) of Bacillus circulans WL-12. The recombinant protein ChtBD-bFGF had the same biological activity as bFGF in promoting fibroblast proliferation. Chitin powder was dissolved in 11 wt% NaOH and 4 wt% urea aqueous solution via the freezing/thawing method. A chitin solution was spread on a glass plate and coagulated with anhydrous alcohol. The chitin binding ability of ChtBD-bFGF was 11.4-fold higher (up to 286 μg/cm2) than bFGF in vitro. The immunofluorescence data indicated that the ChtBD-bFGF@chitin film promoted cell adhesion and proliferation. The ChtBD-bFGF@chitin film and bFGF@chitin films were implanted subcutaneously. Histological analysis showed that ChtBD-bFGF promoted vascularization at the implanted site more effectively than bFGF. These results suggest that the ChtBD-bFGF@chitin film is a stabile delivery vehicle for accelerating wound healing.