Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation
writer：Gao T, Zhang N, Wang Z, Wang Y, Liu Y, Ito Y, Zhang P
keywords：IGF-1; biodegradable PLGA microcarriers; bone tissue engineering; nano-hydroxyapatite; polydopamine
source：期刊
specific source：MacromolBiosci. 2015,15(8):1070-80.(封面文章）
Issue time：2015年
Abstract
In this study, insulin-like growth factor 1 (IGF-1) was successfully immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA microcarriers via polydopamine (pDA). The results demonstrated that the pDA layer facilitated simple and highly efficient immobilization of peptides on the microcarriers within 20?min. Mouse adipose-derived stem cells (ADSCs) attachment and proliferation on IGF-1-immobilized microcarriers were much higher than non-immobilized ones. More importantly, the IGF-1-immobilized PLGA/HA microcarriers significantly increased alkaline phosphatase (ALP) activity and expression of osteogenesis-related genes of ADSCs. Therefore, it is considered that the IGF-1-decorated PLGA/HA microcarriers will be of great value in the bone tissue engineering.
Fulltext: http://dx.doi.org/10.1002/mabi.201500069
Figure 6. CLSM micrographs of PLGA (a), pDA @ PLGA (b), pDA @ PLGA/IGF-1 (c), PLGA/HA (d), pDA @ PLGA/HA (e) and pDA @ PLGA/HA/IGF-1 (f). ADSCs have been cultured on microcarriers for 4 d with actin microfilaments (phalloidin tetramethylrhodamine B isothiocyanate, red) and nucleus (DAPI, blue) staining. All scale bar lengths are 20mm.
