writer：3. Changguo Xue, Yanhua teng, Shangquan Wu, Qingchuan Zhang, Mingxu Zhang
keywords：Microcantilever, Denaturation,Surface stress
source：期刊
specific source：Asian Journal of Chemistry
Issue time：2012年

A real-time detection denaturation of the trypsin using microcantilever sensor platform with optical lever technique was reported. The
trypsin was indirectly immobilized on the cantilever gold surface by thiol self-assembled monolayer and the activated carboxy group of
the thiol by EDC/NHS. Consequently, the deflections of the microcantilever corresponding to the process of injecting different concentration
of GuHCL were generated.