作者：Jiao Ma, Lingjie Song, Hengchong Shi, Huawei Yang, Wei Ye*, Guo Xin, Shifang Luan*, Jinghua Yin, et al.,
关键字：immunoassays, microspheres, hierarchical
论文来源：期刊
具体来源：Journal of Materials Chemistry B, 2018, 6, 3762-3769
发表时间：2018年

Abstract: 

Improving the detection sensitivity of enzyme linked immunosorbent assay (ELISA) is of the utmost importance for meeting the demand of early disease diagnosis. In this work, a sensitive solid substrate for ELISA, i.e., hierarchical iron oxide magnetic microspheres, Fe3O4@mSiO2@poly[poly(ethylene glycol) methacrylate-co-glycidyl methacrylate], was developed via a novel surface-initiated photoiniferter-mediated polymerization (SI-PIMP) strategy. The magnetic microspheres consist of a magnetic Fe3O4 core that gives a high magnetic response, a 3D backbone, a mesoporous SiO2 middle layer, that facilitates microsphere stability and provides anchoring sites, and polymer brushes, that serve as an antifouling and oriented antibody immobilization layer. As a result, the as-prepared microspheres possess a high antibody loading capacity, an enhanced detection signal and a dramatically improved sensitivity, resulting in a 25-fold improvement over conventional ELISA solid substrates.