writer：Jiao Ma, Shifang Luan*, Jing Jin, Jinghua Yin*, et al.,
keywords：polymer brush, immunoassays
source：期刊
specific source：Chemical Communications, 2015, 51, 6749-6752
Issue time：2015年

Abstract:

We propose a facile UV strategy to construct a hierarchically three-dimensional (3D) substrate that comprises a polystyrene (PS) microsphere layer on the cycloolefin polymer (COP) substrate and densely packed hydrophilic polymer brushes grafting from this 3D backbone. This hierarchical substrate gives a high antibody loading capacity and 3D manner of analyte capture, therefore enhancing detection signal while reducing background noise.