50. (Sens. Actuators B Chem.) A label-free aptasensor for turn-on fluorescent detection of ATP based on AIE-active probe and water-soluble carbon nanotubes
作者:Ke Ma, Hui Wang, Haolong Li, Shan Wang, Xing Li, Bin Xu?, Wenjing Tian
关键字:ATP detection, AIE, DNA aptasensor, Water-soluble carbon nanotubes
论文来源:其他
具体来源:Sensors and Actuators B: Chemical 2016, 230, 556-558.
发表时间:2016年
A label-free and turn-on fluorescent ATP detection is reported with a ss-DNA aptamer as the recognition part, an AIE molecule as the fluorescent probe, and water-soluble carbon nanotubes as the selective part with the fluorescent quenching effect. The specific binding between the aptamer and target ATP makes the aptamer released from the water-soluble carbon nanotubes. As a consequence, the AIE-active probes, attaching on the surface of ss-DNA, recover to give fluorescence and present a strong emission. The introduction of water-soluble carbon nanotubes allows creating low background fluorescence, which is a great advantage for ATP detection.
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