writer：Weiqiang Lin,1 Jialin Huang,1 Shuang Guo,? Meijiao Zhao, Xu Chen, Qiuping Shang, Ruoyuan Zhang, Gu
keywords：tunable fluorescent probe
source：期刊
specific source：https://doi.org/10.1039/D3TB00747B
Issue time：2023年
Syphilis, caused by Treponema pallidum (T. pallidum), is associated with the oxidative stress due to its inflammation-like symptom, and detecting the reactive oxygen species (ROS) is crucial for monitoring the infectious process. Herein, we design and synthesize a perylene-based tunable fluorescent probe, PerqdOH, which can detect endogenous O2˙? during T. pallidum infection. The fluorescence peak shifted from 540 nm to 750 nm with increasing O2˙? levels. Besides, both decreased green fluorescence and enhanced red fluorescence could be observed simultaneously during the in vitro infection, providing the real-time monitoring of intracellular O2˙? caused by T. pallidum. Furthermore, the probe exhibited a remarkable signal in the treponemal lesions on the back of a rabbit model. Taken together, our synthesized PerqdOH holds great potential for application in clarifying the infectious process caused by T. pallidum in real time.