作者：Wang, J.; Zhang, L.; Huang, Y.*; Dandapat, A.; Dai, L.; Zhang, G.; Lu, X.; Zhang, J.; Lai, W*.; Chen,T*.
关键字：lateral-flow immunochromatographic test strip
论文来源：期刊
具体来源：Scientific Reports,2017,2045-2322
发表时间：2017年

The probe materials play a significant

role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic

test strip (ICTS). Unlike conventional ICTS assay usually uses single-component,

solid noble metal particles (e.g., gold nanoparticles) as labeled probes, in

our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS

was successfully developed for the detection of clenbuterol (CLE).

The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were

synthesized by varying the volume ratio of [HAuCl4]: [Ag NPs] via the galvanic

replacement reaction. The surface of hollow Ag-Au NPs was functionalized with

11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE

monoclonal antibody to acquire highly stable hollow Au-Ag NPs probe.

Overall size of the Au-Ag NPs, size of the holes within individual NPs and also

Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection

signals and the quantitative data (optical densities) recorded using a bioassay

reader. The sensitivity of optimized hollow Au-Ag NPs

probes for the detection of CLE  has been

achieved even as low as 2 ppb in a short time (within 15 min), which is

superior over the detection performance of conventional test strip using Au NPs.

The optimized hollow

Au-Ag NPs labeled test strip can be used as an ideal candidate

for the rapid screening of CLE in food samples.