writer：Niu Xiaolian, Zhao Liqin, Yin Meng, Huang Di, Wei Yan, Hu Yinchun, Chen Weiyi
keywords：calcium chloride, electrospun polyamide66, mineralization, mechanical properties, cytocompatibility
source：期刊
specific source：Tissue engineering Part C Metholds
Issue time：2020年
This study aimed at designing a novel electrospun scaffolding material that structurally and chemically resembles native extracellular matrix (ECM) for bone tissue engineering. Calcium chloride-complexed polyamide66 (PA66/CaCl2) and pure PA66 electrospun nanofibers were fabricated by electrospinning method. The scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) were used to investigate the effect of the presence of ionized salt in the polymer solution on the mechanical properties and other properties of the electrospun scaffolds. The results show that addition of CaCl2 to PA66 solution can achieve an internal modification and improve tensile strength and modulus of the polymeric electrospun nanofiber. Ca2+ be uploaded through electrospun fibers could provide nucleation sites for the formation of hydroxylapatite (HA) coating. After mineralization, the tensile strength and modulus of HA/PA66/CaCl2 scaffolds reach up to 41.33 ± 16.17 MPa and 168.59 ± 42.20 MPa, respectively. LIVE/DEAD assay shows that MC3T3-E1 cells could adhere and grow on the surface of samples. Cell Counting Kit-8 (CCK-8) results indicate that HA/PA66/CaCl2 displays a more favorable ability to promote MC3T3-E1 cell proliferation and growth than that of the other groups with the prolongation of culture time. These results demonstrate that HA/PA66/CaCl2 scaffolds that structurally and chemically resemble native bone have a good cytocompatibility, might be a potential candidate for bone tissue engineering.