writer：Ruofan Wang,# Jiangying Zhu,# Guohua Jiang,* Yanfang Sun, Liming Ruan,* Pengfei Li, Haoyan Cui
keywords：bioadhesives, wound dressings, wound closure, chitosan, silk fibroin
source：期刊
specific source：ACS Applied Biomaterials
Issue time：2020年

Abstract: Wound dressing have been used for decades to be effective for accelerating skin wound healing. However, practical applications are still limited due to their lower cell affinity, tissue adhesiveness and biocompatibility. Natural polymers are the important biomaterials because of their excellent biodegradability, biocompatibility and low immungenicity. In this work, the composite bioadhesives (PLS-CS/RSF) were prepared from regenerated silk fibroin (RSF) and polylysine-modified chitosan (PLS-CS) that corss-linked by Ca2+ ions. The adhesion property tests showed the PLS-CS/RSF exhibited excellent bonding potentials for various substrates, and the adhesive strength was up to 70 KPa for isolated porcine skin by the extension test. The as-prepared PLS-CS/RSF were nontoxic, displayed obvious antibacterial effects against S. aureus and E. coli in vitro, and their bacteriostasis rates were 100% after 120 min treatment. In addition, the PLS-CS/RSF exhibited favorable cytocompatibility by CCK-8 assay. Animal model of wound closure results showed that PLS-CS/RSF can promote wound closure and the integrity of wound healing, inhibiting the secretion of inflammatory factor (IL-6) and tumor necrosis factor (TNF-α), and stimulating vascular factor (CD31) and α-smooth muscle actin (α-SMA) to release of vascular growth factor and promote angiogenesis during the process of wound healing by immunohistochemical assay.