writer：Minh Khanh Nguyen, Cong Truc Huynh, Guang Hui Gao, Ji Hyun Kim, Dai Phu Huynh, Su Young Cha
keywords：hydrogels
source：期刊
specific source：Soft Matter
Issue time：2011年

An injectable biodegradable hydrogel system based on oligo(amidoamine/b-amino ester) (OAAAE)

was designed and synthesized for controlled release of insulin under the physiological conditions.

OAAAE was prepared in one step via the Michael-addition oligomerization of the secondary amine

groups of 4,4-trimethylene dipiperidine (TMDP) with the vinyl groups of 1,8-octylene diacrylamide

(ODA) and 1,6-hexane diol diacrylate (HDA). The formed oligomer was characterized by 1H NMR

and gel permeation chromatography (GPC). OAAAE in aqueous solution (20 wt%) underwent a gel–

sol transition in the pH range of 6.8–7.4. A complex hydrogel was formed when mixing insulin with the

oligomer solution followed by increasing pH and temperature. Degradation of the hydrogel, influence

of insulin on gel phase and gel strength, in vitro cytotoxicity of OAAAE, in vitro and in vivo release of

insulin from the complex hydrogel were investigated. Furthermore, the in vivo release profile of insulin

from the complex hydrogel was compared with that from the neutral hydrogel.