writer：Ruixia Hou, Lei Nie, Gaolai Du, Xiaopeng Xiong, and Jun Fu*
keywords：polysaccharides, magnetic, hydrogels
source：期刊
specific source：Colloids and Surface B: Biointerfaces
Issue time：2015年

This paper aims to investigate the synergistic effects of natural polysaccharides and inorganic nanoparticles on cell adhesion and growth on intrinsically cell non-adhesive polyvinyl alcohol (PVA) hydrogels. Previously, we have demonstrated that Fe₂O₃ and hydroxyapatite (nHAP) nanoparticles are effective in increasing osteoblast growth on PVA hydrogels. Herein, we blended hyaluronic acid (HA) and chondroitin sulfate (CS), two important components of cartilage extracellular matrix (ECM), with Fe₂O₃/nHAP/PVA hydrogels. The presence of these natural polyelectrolytes dramatically increased the pore size, and the equilibrium swelling ratio (ESR) while remaining excellent compressive strength of hydrogels. Chondrocytes were seeded and cultured on composite PVA hydrogels containing Fe₂O₃, nHAP and Fe₂O₃/nHAP hybrids and Fe₂O₃/nHAP with HA or CS. Confocal laser scanning microscopy (CLSM) and cell counting kit-8 (CCK-8) assay consistently confirmed that the addition of HA or CS promotes chondrocyte adhesion and growth on PVA and composite hydrogels. Particularly, the combination of HA and CS exhibited further promotion to cell adhesion and proliferation compared to any single polysaccharide. The results demonstrated that the magnetic composite nanoparticles and polysaccharides provided synergistic promotion to cell adhesion and growth. Such polysaccharide-augmented composite hydrogels may have potentials in biomedical applications.

 

http://www.sciencedirect.com/science/article/pii/S0927776515003057