writer：Lei Nie; Guohua Zhang; Ruixia Hou; Haiping Xu; Yaping Li; Jun Fu
keywords：Chondrocyte, Hydrogels, Controlled Release, TGF-beta1, PLGA Microspheres
source：期刊
specific source：Colloids ana Surfaces B: Biointerface, 2015, 125, 51-57
Issue time：2015年
Poly(vinyl alcohol) (PVA) hydrogels have been candidate materials for cartilage tissue
engineering. However, the cell non-adhesive nature of PVA hydrogels has been a limit. In this paper, the cell adhesion and growth on PVA hydrogels were promoted by compositing with transform growth factor-beta 1 (TGF-beta 1) loaded porous poly(D,L-lactide-co-glycolide) (PLGA) microspheres with controlled release profiles. The porous microspheres were fabricated by a modified double emulsion method with bovine serum albumin (BSA) as porogen. The average pore size of microspheres was manipulated by changing the BSA/PLGA ratio. Such controllable porous structures effectively influenced the encapsulation efficiency (Eencaps) and the release profile of TGF-beta 1. By compositing PVA hydrogels with such TGF-beta 1-loaded PLGA microspheres, chondrocyte adhesion and proliferation were significantly promoted in a controllable manner, as confirmed by fluorescent imaging and quantitative CCK-8 assay. That is, the chondrocyte proliferation was favored with a higher Eencaps of TGF-beta 1 in microspheres or with higher microsphere contents in the hydrogels. These results demonstrated a facile method to improve the cell adhesion and growth on the intrinsically cell non-adhesive PVA hydrogels, which may find applications in cartilage substitution.