writer：Jingjing Zhang, Shizhi Zhang, Chaoqun Niu, Chen Liu, Jie Du*, and Yong Chen
keywords：ATP detection, label-free fluorescence, enzyme-free, gold nanoparticles, DNA calculator
source：期刊
specific source：Molecules 2018, 23, 2494
Issue time：2018年

Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of

the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M0) as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50–500 nmol/L (R2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.