writer：Xi Cui, Youju Huang,* Jingyun Wang, Lei Zhang, Yun Rong, Weihua Lai* and Tao Chen*
keywords：Au NPs, Polymer Brushes, lateral flow immunoassay, Escherichia coli O157:H7
source：期刊
specific source：RSC Advances, 2015, 5, 45092–45097
Issue time：2015年
Due to the distinctive features of ease-of-use, low-cost and portable detection, gold nanoparticles (AuNPs) based lateral flow immunoassay (LFA) is an effective and currently used method for the detection of Escherichia coli O157:H7 (E. coli O157:H7), however, its low sensitivity limits its practical wide-of-use. In our present study, we have systematically optimized the size and uniformity of AuNPs to maximally amplify both visual inspection signals (the color of test line) and quantitative data (light intensity) recorded by ‘Assay Reader’ instrument. The remarkable enhancement of detection sensitivity can be achieved to 10² colony-forming units (CFU)/mL by taking advantages of the optimized AuNPs and the separated incubation of AuNPs/antibody/E. coli O157:H7 complex. Quantitative detection of E. coli O157:H7 was obtained partially in a wide concentration range with good repeatability. The optimized newly AuNPs-based LFA is well suited for fast quantitative and qualitative food analysis.