作者：Feng Wang, Xu Li, Furong Zhang, Xiaoyang Liu, Pengcheng Hu, Tamás Beke-Somfai, Xiaolin Lu*
关键字：Interface, Lipid Hydrolysis, Phospholipase A1, SFG
论文来源：期刊
具体来源：Langmuir, 2019 3539, 12831-12838
发表时间：2019年

The interfacial hydrolysis of phospholipids catalyzed by phospholipase A1 (PLA1) was studied via sum frequency generation (SFG) vibrational spectroscopy and fluorescence microscopy. Both monolayer and bilayer setups were used to confirm the hydrolysis mechanism. During the hydrolysis, lysophospholipids, one of the hydrolysis products, were desorbed from the interface into the solution, while the other products, fatty acids, self-organized and accumulated with PLA1 at the interface to form the PLA1-induced regions, which can serve as nonspecific binding domains for proteins and thus lead to human vascular diseases. This experimental study provides the essential information on revealing the interfacial biochemical process related to the metabolism of the lipids, which is one of the basic building blocks for cells.

https://pubs.acs.org/doi/10.1021/acs.langmuir.9b02284