The husbandry pattern of yak meat is a crucial factor for its market price and competitiveness; deliberately mislabeling of the production information will cause consumer confidence crisis. In this work, stable isotope signatures of free-range grass-fed (FRG) and captive grain-fed (CG) yak meat were analyzed for their discrimi-nation. & delta;13C, & delta;2H, & delta;18O, & delta;15N, & delta;34S of bulk muscle and & delta;13C of six fatty acids were respectively determined by EA-IRMS and GC-C-IRMS. ANOVA and correlation analysis of stable isotope ratios of yak muscle and fatty acids against its feed indicated that their & delta;13C, & delta;15N, & delta;34S significantly are related, which provides the evidence of diet and trophic information. & delta;13C of six fatty acids (FAs) are significantly different (p < 0.05) between two patterns; all FAs of FRG yak meat are highly related to grass silage (R > 0.70), but saturated and unsaturated FAs of CG yak meat are respectively related to soybean silage (R > 0.70) and maize silage (R > 0.56). PLS-DA and LDA modeling based on stable isotope ratios of yak muscle and FAs together discriminate yak meat from these two husbandry patterns with accuracies of 100 %. This strategy may be promising as a feasible method for confirming the husbandry patterns of yak meat, ensuring label authenticity and food safety.