Determination of Molecular Weight of Silk Fibroin by Non-Gel Sieving Capillary Electrophoresis

Determination of Molecular Weight of Silk Fibroin by Non-Gel Sieving Capillary Electrophoresis
来源：张耀鹏研究员个人网站 2010-03-29

A simple non-gel sieving capillary electrophoresis (NGSCE) method was established to determine molecular weight (MW) of silk fibroin using capillary electrophoresis system (CE). The background electrolyte with a pH of 8.8 was based on the three components polyethylene glycol, tris(hydroxymethyl)aminomethane (Tris) and sodium dodecyl sulfate (SDS). NGSCE showed a good linear relationship with fine reproducibility between the migration time and the MW of standard proteins. It was found that the regenerated silk fibroin had a MW around 83 kDa with a wide molecular weight distribution (MWD). This absolute value is lower than the result obtained from the Lauryl sodium sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) due to their different principles, but their similar MWD shapes indicated that NGSCE could be a feasible, highly sensitive, rapid method for the determination of MW of silk fibroin.
[来源：中国聚合物网]