Gold Janus nanomaterials have a tremendous significance for the novel bifunctional materials, significantly expanding the application scope of gold nanomaterials, especially Janus gold-thiol coordination polymer due to their exceptional biological characteristics, stability, plasmon effect, etc. The recent research on Janus gold nanoparticles and monolayer films of preparation and application has been summarized and in this review. To begin, we briefly introduce overview of Janus nanomaterials which received intense attention, outline current research trends, and detail the preparation and application of gold nanomaterials. Subsequently, we present comprehensively detailing fabrication strategies and applications of Janus gold nanoparticles. Additionally, we survey recent studies on the Janus gold nano-thickness films and point out the outstanding advantage of application on the tunable surface plasmon resonance, high sensitivity of surface-enhanced Raman scattering and electrical analysis fields. Finally, we discuss the emerging trends in Janus gold nanomaterials and address the associated challenges, thereby providing a comprehensive overview of this area of research.